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Laboratoire Commun de Microbiologie IRD-ISRA-UCAD

Thématiques de Recherche
  • Biotechnologies végétales : Micropropagation et embryogenèse somatique d’espèces maraîchères, horticoles, agro-forestières.
  • Biologie moléculaire : caractérisation moléculaire, analyse de diversité génétique
  • Biologie de la reproduction
Responsable : Pr. Ibrahima Ndoye
Adresse de Correspondance  :
Laboratoire Commun de Microbiologie (LCM)
IRD / ISRA / UCAD Campus de Bel-Air, Bâtiment B3
Rte des hydrocarbures,
BP 1386 - CP 18524
Téléphone : (221) 849 33 18
Fax : (221) 849 33 02
E.mail :
Site Web :


Fatou GUEYE, Godar SENE, Mansour GNING, Nathalie DIAGNE, Arsène SANON, Fatou NDOYE, Nirina RAKOTOARIMANGA, Abdoulaye SOUMARE, Mlle N. BENKHOUA, Mlle H. KCHAKECH., Niokhor BAKHOUM.

  • Diouf D, Samba-Mbaye R, Lesueur D, Ba AT, Dreyfus B, Neyra M (2007). Genetic diversity of /Acacia seyal /Del. rhizobial populations indigenous to Senegalese soils in relation to salinity and pH of the sampling sites. 15e Congrès International pour la Fixation Biologique de l’Azote & 12e Congrès de l’Association Africaine pour la fixation Biologique de l’Azote, 21–26 Janvier 2007, Cape Town, Afrique du Sud
  • Ramatoulaye Thiaba Samba, Marc Neyra, Dorego Francis, Samba Sylla, Ibrahima Ndoye (2007). Diversity and competitivity of rhizobia of legume tree Acacia nilotica in Senegal.15e Congrès International pour la Fixation Biologique de l’Azote & 12e Congrès de l’Association Africaine pour la fixation Biologique de l’Azote, 21–26 Janvier 2007, Cape Town, Afrique du Sud.
  • L. Fougnies, S. Renciot, F. Muller, C. Plenchette, Y. Prin, S.M. de Faria, J.M. Bouvet, S.Nd. Sylla, B. Dreyfus, I. Ndoye, A.M. Bâ. 2007. Arbuscular mycorrhizal colonization and nodulation improve flooding tolerance in Pterocarpus officinalis Jacq. Seedlings. Capetown, janvier 2007.
  • Ben Romdhane S., H. Nasr, Samba-Mbaye, M. Neyra, M.H. Ghorbal and P. De Lajudie - 2006 - Genetic diversity of Acacia tortilis ssp. raddiana rhizobia in Tunisia assessed by 16S and 16S-23S rDNA genes analysis. In : Journal of Applied Microbiology.,
  • Ouahmane, L., Duponnois, R., Hafidi, M.,Kisa, A., Boumezouch, M., Thioulouse, J.Plenchette, C. In : Plant Ecology (2006) - Some Mediterranean plant species (Lavandula spp.and Thymus satureioides ) act as potential ‘plant nurses ’for the early growth of Cupressus atlantica.
  • Ramanankierana, N., Rakotoarimanga, N., Thioulouse, J., Kisa, M., Randrianjohany, E., Ramaroson, L., and Duponnois, R. - 2006 - The ectomycorrhizosphere effect influences functional diversity of soil microflora. In : International Journal of Soil Sciences (1):8-19.
  • Krasova-Wade, T., Diouf, O., Ndoye I., Sall, C.E., Braconnier, S. & Neyra, M. 2006. Water-condition effects on rhizobia competition for cowpea nodule occupancy. Afr. J. Biotech. 5 (16) :1457-1463.
  • ANDRE, S, GALIANA, A., LE ROUX, C. PRIN, Y., NEYRA, M., R. DUPONNOIS. - Ectomycorrhizal symbiosis enhanced the efficiency of inoculation with two Bradyrhizobium strains and Acacia holosericea growth. Mycorrhiza (2005) 15: 357-364.
  • Assigbetse, K., Gueye, M., Thioulouse, J., Duponnois, R. - Soil Bacterial Diversity Responses to Root Colonization by an Ectomycorrhizal Fungus are not Root-Growth-Dependent - In : Microbial Ecology. 1-10 – 2005.
  • Badiane, S., Ndoye, I., Gueye, M. – Estimating N by maize (Zea mays L.) from multipurpose tree scies using a 15N isotope technique – In : Revue Sénégalaise des Recherches Agricoles et Agroalimentaires. 2005. Vol. 1, n°00 pp.41-47.
  • Ben Romdhane,S., Nasr,H., Samba-Mbaye,R., Neyra,M., Ghorbal, M.H. - Diversity of Acacia tortilis rhizobia revealed by PCR/RFLP on crushed root nodules in Tunisia . In : Annals of Microbiology,55 (4)249-258 (2005).
  • Diédhiou, A.G., Guèye, O., Diabaté, M., Prin, Y.,. Duponnois, R. Dreyfus, B., A. M. Bâ - Contrasting responses to ectomycorrhizal inoculation in seedlings of six tropical African tree scies. In : Mycorrhiza:1–7, 2005.
  • Diouf, D., Duponnois, R., Ba, A.T., Neyra, M., and Lesueur, D. – 2005 – Symbiosis of Acacia auriculiformis and Acacia mangium with mycorrhizal fungi and Bradyrhizobium spp. Improuves salt tolerance in greenhouse conditions. In : Functional Plant Biology, 32, p. 1143-1152.
  • Duponnois, R. - Bacteria Helping Mycorrhiza Development - In : Soil Biology, Vol. 7 – 2005.
  • Duponnois, R., Colombet, A., Hien, V., Thioulouse, J. The mycorrhizal fungus Glomus intraradices and rock phosphate amendment influence plant growth and microbial activity in the rhizosphere of Acacia holosericea - Soil Biology & Biochemistry 37 (2005) 1460–1468.
  • Duponnois, R., Founoune, H., Masse, D., Pontanier, R. Inoculation of Acacia holosericea with ectomycorrhizal fungi in a semiarid site in Senegal: growth response and influences on the mycorrhizal soil infectivity after 2 years plantation - In : Forest Ecology and Management 207 (2005) 351–362
  • Duponnois, R., Paugy, M., Thioulouse, J., Masse, D, .Lepage, M. - Functional diversity of soil microbial community, rock phosphate dissolution and growth of Acacia seyal as influenced by grass-,litter- and soil-feeding termite nest structure amendements - In : Geoderma 124 (2005) 349–361
  • Faye, A., Sarr, A., Lesueur, D. - Effect of inoculation with rhizobia on the Gum-arabic production of 10-year-old Acacia-senegal trees. In : Arid Land Research and Management20 :1-7, 2005-2006.
  • Lesueur, D, Faye, A., Duponnois, R., Diouf, D., Plenchette, C.,Sougoufara, B. – Acacia mangium for a sustentateur wood production in the irrigated areas of the soudano-sahelian countries. In : Improvement and Culture of nitrogen Fixing Trees – Vol. 8 n° 1 - 2005 –
  • Lesueur, D., Duponnois, R. - Relations between rhizobial nodulation and root colonization of Acacia crassicarpa provenances by an arbuscular mycorhize fungus, Glomus intraradices Schenk and Smith or an ectomycorrhizal fungus, Pisolithus tinctorius Coker & Couch - Ann. For. Sci. 62 (2005) 467–474
  • Ndiaye, F.,Diop, T.A.,Sy, M.O.,Anicet, G.B.,Sow, H.A.,Ba, T.A. - Mycorhization contrôlée de plantes micropropagées de pomme de terre (Solanum tuberosum L.) In : Revue Sénégalaise des Recherches Agricoles et Agroalimentaires – 2005 pp. 33-40
  • Plenchette, C., Duponnois, R. - Growth response of the saltbush Atriplex nummularia inoculation with the arbuscular mycorrhizal fungus Glomus intraradices. In : Journal of Arid Environments 61 (2005)535 –540
  • Sane, D., Ould Kneyta, M., Diouf, D.,, Diouf, D.,Badiane, F.A., , Sagna, M., And Borgel A. Growth and development of date palm (Phoenix dactylifera L.) seedlings under drought and salinity stresses, African Journal of Biotechnology Vol. 4 (9), pp. 968-972, September 2005
  • Sanon, A., Martin, P., Thioulouse, J., Plenchette, C. Spichiger, R., Lepage, M., Robin Duponnois, D. - Displacement of an herbaceous plant species community by mycorrhizal and non-mycorrhizal Gmelina arborea, an exotic tree, grown in a microcosm expriment In : Mycorrhiza : 2005.
  • Sarr, A., Faye, A., Oihabi, A., Houeibib, M.A.J.O., Neyra, M., Lesueur, D. – Inoculation en station et au champ d’Acacia sénégal avec des souches sélectionnées de Rhizobium. In : Bois et Forêts des Tropiques, 2005, n° 283. pp.6-18
  • Sarr, A., Neyra, M., Ould Houeibib, M.A.,Ibrahima Ndoye, Oihabi, A.and Didier Lesueur, Rhizobial Populations in Soils from Natural Acacia senegal and Acacia nilotica Forests in Mauritania and the Senegal River Valley. In : Microbial Ecology. 1-11,2005.
  • Sarr., A., Diop., B., Peltier, R., Neyra, M., Lesueur, D. – 2005 - Effect of rhizobial inoculation methods and host plant provenances on nodulation and growth of Acacia senegal and Acacia nilotique. In.: New Forests, 29:75–87.
  • Ba, A.M., Samba, R., Sylla, S.N., Le Roux, C., Neyra, M., Rousteau, Imbert, D. And Toribio.- 2004 - Caractérisation de la diversité des microorganismes symbiotiques de Pterocarpus officialis dans des forêts marécageuses de Guadeloupe et Martinique. Rev. Ecol. Vol. 59.
  • Demba Diallo M.; Martens M.; Vloemans N.; Cousin S.; Van Dekerckhove T.T.M.; Neyra M.; De Lajudie P.; Willems A.; Gillis M.; Vyverman W.; Van Der Gucht K.- Phylogenetic Analysis of Partial Bacterial 16S rDNA Sequences of Tropical Grass Pasture Soil under Acacia tortilis subsp. raddiana in Senegal. Systematic and Applied Microbiology, 1 March 2004, vol. 27, iss. 2, pp. 238-252(15)
  • Demba-Diallo, M., Willem, A., Vloemans, N., Cousin, S., Vandekerckhove, T.T., De Lajudie, P., Neyra, M., Vyverman, W., Gillis, M., Van Der Gucht K. – 2004 – Polymerase chain reaction denaturing gradient gel electrophoresis analysis of the N2-fixing bacteria diversity in soil under Acacia tortilis spp. Raddiana and Balanites aegyptiaca in the dry land part of Senegal In : Environmental Microbiological 6:4, 400-415.
  • Diedhiou , A.G., Verpillot, F., Gueye, O., Dreyfus, B., Duponnois, R., Ba , A.M. – 2004 - Do concentrations of glucose and fungal inoculum influence the competitiveness of two early-stage ectomycorrhizal fungi in Afzelia africana seedlings? Forest Ecology and Management 204 (2004) 187-194
  • Diedhiou, A.G., Ba, A.M., Sylla, S.Nd., Dreyfus, B., Neyra, M., Ndoye, I. – 2004 - The early-stage ectomycorrhizal Thelephoroid fungal competitive and effective on Afzelia africana nursery conditions in Senegal Mycorrhiza 14:313 –322.
  • Djigal, D., Brauman, A., Diop, T.A., Chotte, J.L.,Villenave, C. 2004 - Influence of bacterial-feeding nematodes (Cephalobidae) on soil microbial communities during maize growth Soil Biology & Biochemistry 36 (2004) 323–331.
  • Guene, N.F.D., Diouf, A., Gueye, M. – 2004 – Nitrogen fixation in the common bean (Phaseolus vulgaris) – A multiplicatrice inoculation trial in Senegal (ed) SERRAJ R. In Oxford & IBH Publishing Co. Pvt.Lt. New Delhi. 247-252
  • Thiao, M, M.,Neyra, M.,Isidore, E.,Sylla, S.,and Lesueur, D. - Diversity and effectiveness of rhizobial strains from Gliricidia sepium native to Reunion Island,Kenya and New Caledonia. In : World Journal of Microbiology &Biotechnology 20 :703 –709,2004.
  • Andre, S., M. Neyra And Duponnois, R. (2003). "Arbuscular Mycorrhizal Symbiosis Changes the Colonization Pattern of Acacia tortilis spp. Raddiana Rhizosphere by Two Strains of Rhizobia." Microbial Ecology 45: 137-144.
  • Anonymes (2003) Evaluation and use of the biodiversité of the microsymbionts of Calliandra calothyrsus to optimise fodder production within small-scale farming systems in the humid zone. Eds POTTINGER, A.J., LESUEUR, D. (E Projet n° IC18-CT97-0194.
  • De Lajudie, P., B. Dreyfus, et al. (2003). “ Diversité taxonomique et propriétés symbiotiques des rhizobium nodulant Acacia raddiana au nord et au sud du Sahara.” Un arbre au désert Acacia raddiana dans les Editions IRD: 145-170.
  • De Lajudie, P., Dreyfus, B., Boivin, C., Ba, S., Ndiaye, A., Lorquin, J., Neyra, M., Detrez, C., Willems, A., Gillis, M., Jeder, H., Prome, J.C. – 2003 – Diversité taxonomique et propriétés symbiotiques des rhizobium nodulant Acacia raddiana au nord et au sud du Sahara. In : Un arbre au désert Acacia raddiana dans les Editions IRD.
  • Dione, Y., Sy,O., Diop, T. 2003 – Réponse d’Allocasuraina verticillata (Lam) L. Johnson et de Casuarina equisetifolia (L.) à l’inoculation mycorhizienne arbusculaire au Journal de la Faculté des Sciences et Techniques UCAD, Dakar 2 (3).
  • Diop, A.T. – 2003 - In vitro culture of arbuscular mycorrhizal fungi:advances and future prospects African Journal of Biotechnology Vol. 2 (12), pp. 692-697.
  • Diop, A.T., Krasova-Wade, T., Diallo, A., Diouf, M., Gueye, M.- 2003 - Solanum cultivar responses to arbuscular mycorrhizal fungi: growth and mineral status 1,2 African Journal of Biotechnology Vol. 2 (11), pp. 429-433.
  • Diop, T. – 2003 Influence de la mycorhization arbusculaire sur la culture intensive de l’oignon (Allium cepa L.) au Sénégal « au Journal de la Faculté des Sciences et Techniques UCAD.
  • Diouf, D., Forestier, S., Neyra, M., Lesueur, D. – 2003 – Optimisation of inoculation of Leucaena leucocephala and Acacia mangium with rhizobium under green house conditions. Ann. For. Sci. 60 (2003) 379-384.
  • Diouf, D., S. Forestier, et al. (2003). “Optimisation of inoculation of Leucaena leucocephala and Acacia mangium with rhizobium under greenhouse conditions.” Ann. For. Sci. 60: 379-384.
  • Diouf, M., Neyra, M., Grouzis, M. – 2003 – Phénologie de la nodulation d’Acacia raddiana en milieu naturel. In : Un arbre au désert Acacia raddiana dans les Editions IRD.
  • Diouf. D., Diop, T.A And Ndoye, I. - 2003 - Actinorhizal, mycorhizal and rhizobial symbioses: how much do we know? In : African Journal of Biotechnology. Vol. 2 (1), pp. 1-7.
  • Fall, L., Diouf, D., Fall-Ndiaye, M.A., Badiane, F.A., And Gueye, M. - 2003 - Genetic diversity in cowpea [Vigna unguiculata (L.) Walp.] varieties determined by ARA and RAPD techniques. In : African Journal of Biotechnology Vol. 2 (2), pp. 48-50, February 2003.
  • Gueye, M. Ndoye, I. – 2003 – Le potentiel d’azote d’Acacia raddiana comparé à celui d’Acacia senegal, Acacia seyal et Faidherbia albida. In : Un arbre au désert Acacia raddiana dans les Editions IRD.
  • Krasova-Wade,T., Ndoye,I., Braconnier, S., Sarr, B., De Lajudie, And Neyra, M. - 2003 - Diversity of indigeneous bradyrhizobia associated with three cowpea cultivars (Vigna unguiculata (L.) Walp.) grown under limited and favorable water conditions in Senegal (West Africa). In : African Journal of Biotechnology Vol. 2 (1), pp. 13-22.
  • Manga, A., Diop, T. – 2003 – Recherche au Sénégal : Des champignons endomycorhiziens pour aider les plantes à supporter le stress salin ? In : Info Agri Afrique n° 001 Janvier-Février 2003, pp. 20-22
  • Ndiaye, F., Diop, T., Sy, Mo., Manga, A., Sow, H.A., Diallo, A., Ba, A.T. – 2003 – Effects Glomus aggravation sur le développement ex vitro de plantes microprogrammations de pomme de terre (solanum tuberosum L.) var. bintje) en présence de phosphate naturel du Sénégal. In : Annales de l’Université de Ouagadougou, série, C, vol. 001, pp.46-56.
  • Ba, S., A. Willems, et al. (2002). “Symbiotic and taxonomic diversity of rhizobia isolated from Acacia tortilis subsp. raddiana in Africa.” System. Appl. Microbiol. 25: 130-145.
  • Diouf, D., B. Sougoufara, et al. (2002). “Le reboisement au Sénégal : bilan des réalisations de 1993 à 1998.” Rev. For. Fr. LIV: 227-238.
  • Duponnois, R., H. Founoune, et al. (2002). “Influence of the controlled dual ectomycorrhizal and rhizobal symbiosis on the growth of Acacia mangium provenances, the indigenous symbiotic microflora and the structure of plant parasitic nematode communities.” Geoderma 109: 85– 102.
  • Founoune, H., Duponnois R., et al. (2002). “Ectomycorrhization of Acacia mangium, Willd.and Acacia holosericea, A. Cunn. ex G. Don in Senegal. Impact on plant growth, populations of indigenous symbiotic microorganisms and plant parasitic nematodes.” Journal of Arid Environments 50: 325-332.
  • Founoune, H., R. Duponnois, et al. (2002). “Interactions between ectomycorrhizal symbiosis and fluorescent pseudomonads on Acacia holosericea :isolation of mycorrhiza helper bacteria (MHB)from a Soudano-Sahelian soil.” FEMS Microbiology Ecology 41: 37-46.
  • Founoune, H., R. Duponnois, et al. (2002). “Mycorrhiza helper bacteria stimulate ectomycorrhizal symbiosis of acacia holosericea with pisolithus alba.” New Phytologist 153: 81-89.
  • Germani, G., La Massese, C.S. - 2002 - Description of four new species and two populations of Rotulenchus (Nematoda : hoplolaimidae) In : Nematol. Medit. Vol. 30 : 203-208.
  • Lesueur, D., Duponnois R., Diouf D., Plenchette, C., Sougoufara, B. - (2002) – Introductions réussies d’espèces d’acacias australiens à croissance dans les périmètres irrigués situés au Nord du Sénégal: perspectives pour les pays d’Afrique de la zone soudano-sahélienne In : Recherche / Développement n° 32 Janvier-Mars.
  • Lesueur, D., Duponnois R., et al. (2002). “Introductions réussies d’espèces d’acacias australiens à croissance dans les périmètres irrigués situés au Nord du Sénégal: perspectives pour les pays d’Afrique de la zone soudano-sahélienne.” Recherche / Développement 32 (Janvier-Mars).
  • Ndiaye, A. A., S. N. Sylla, et al. (2002). “Utilisation de la technique d'électrophorèse des protéines totales sur gel de polyacrylamide-SDS (SDS-PAGE) pour l'étude de la diversité des rhizobiums d'Acacia tortillis (Forsk.) Hayne subsp. raddiana (Savi) Brenan.” African Journal of Science and Technology 3(1).
  • Neyra, M., R. Duponnois, et al. (2002). “Incidence de meloidogyne incognita et de M. javanica sur la végétation et l'appareil symbiotique de Sesbania rostrata.” Nematol. Medit. 30: 97-101.
  • Odee, D. W., S. A. Indieka, et al. (2002). “Evaluation of inoculation procedures for Calliandra calothyrsus Meisn. grown in tree nurseries.” Biol Fertil Soils 36: 124-128.
  • Rouland-Lefevre, C., M. N. Diouf, et al. (2002). “Phylogenetic Relationships in Termitomyces (Family Agaricaceae) Based on the Nucleotide Sequence of ITS: A First Approach to Elucidate the Evolutionary History of the Symbiosis between Fungus-Growing Termites and Their Fungi.” Molecular Phylogenetics and Evolution 22(3): 423-429.
  • Samba, R. T., S. N. Sylla , et al. (2002). “Biological nitrogen fixation in Crotalaria species estimated using the 15 N isotope dilution method.” African Journal of Biotechnology Vol. 1((1)): 17-22.
  • Sylla, S. N., Ndoye I., et al. (2002). “Estimates of biological nitrogen fixation by Pterocarpus lucens in a semi arid natural forest park in Senegal using 15 N natural abundance method.” African Journal of Biotechnology 1(2): 50-56.
  • Sylla, S. N., R. T. Samba, et al. (2002). "Phenotypic and Genotypic Diversity of Rhizobia Nodulating Pterocarpus erinaceus and P. lucens in Senegal." System. Appl. Microbiol 25: 572-583
  • Sylla, S. N., R. T. Samba, et al. (2002). “Phenotypic and Genotypic Diversity of Rhizobia Nodulating Pterocarpus erinaceus and P. lucens in Senegal.” System. Appl. Microbiol 25: 572–583.
  • Ba, A., T. Guissou, et al. (2001). “Mycorhization controlée et fertilisation phosphatée : application à la domestication du jujubier.” Fruits 56(4): 261-269.
  • Diagne, O., K. Ingleby, et al. (2001). “Mycorrhizal inoculum potential of soils from alley cropping plots in Senegal.” Forest Ecology and Management 146: 35-43.
  • Diouf, D., M. Neyra, et al. (2001). “Le Plan d'Action Forestier du Sénégal : Bilan et Perspectives des Actvités de Reboisement de 1993 à 1998, (270(A).” Bois et Forêts des Tropiques n° 270(4).
  • Diouf, D., S. Forestier, et al. (2001). “Optimisation of inoculation of Calliandra calothyrsus, leucaena leucocephala and Acacia mangium with rhizobia.” In : 13th International Congress oon Nitrogen Fixation, Hamilton, Ontario, Canada, July 2-7 (poster).
  • Forestier, S., G. Alvarado, et al. (2001). “Effect of Rhizobium inoculation methodologies on nodulation and growth of leucaena leucocephala.” World Journal of Microbiology & Biotechnology 17: 359-362.
  • Lesueur, D., K. Ingleby, et al. (2001). “Improvement of forage production in Calliandra calothyrsus: methodology for the identification of an effective inoculum containing rhizobium strains and arbuscular mycorrhizal isolates.” Journal of Biotechnology 91: 269-282.
  • Wade, T., I. Ndoye, et al. (2001). “Diversity of Bradyrhizobium nodulting the cowpea (Vigna unguiculata (L.) in Senegal (West Africa).” In 13 th International Congress on Nitrogen Fixation, Hamilton, Ontario, July 2-7, 2001.